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scanning densitometry  (Bio-Rad)


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    Bio-Rad scanning densitometry
    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by <t>densitometry</t> scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
    Scanning Densitometry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Bromodomain-Containing 4 Is a Positive Regulator of Interleukin-34 Production in the Gut"

    Article Title: Bromodomain-Containing 4 Is a Positive Regulator of Interleukin-34 Production in the Gut

    Journal: Cells

    doi: 10.3390/cells13201698

    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
    Figure Legend Snippet: BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.

    Techniques Used: Inhibition, Expressing, Transfection, Control, Real-time Polymerase Chain Reaction, Western Blot



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    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by <t>densitometry</t> scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
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    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by <t>densitometry</t> scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
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    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by <t>densitometry</t> scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
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    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by <t>densitometry</t> scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
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    Image Search Results


    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.

    Journal: Cells

    Article Title: Bromodomain-Containing 4 Is a Positive Regulator of Interleukin-34 Production in the Gut

    doi: 10.3390/cells13201698

    Figure Lengend Snippet: BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.

    Article Snippet: Computer-assisted scanning densitometry (Image-Lab 5.2.1, Bio-Rad Laboratories, Milan, Italy) was used to analyze the intensity of the immunoreactive bands.

    Techniques: Inhibition, Expressing, Transfection, Control, Real-time Polymerase Chain Reaction, Western Blot